두경부암세포에서 hydroxibacteriochlorin을 이용한 in vitro 광역학치료
In vitro photodynamic therapy using hydroxybacteriochlorine on head and neck cancer cell
오충훈, 이정구, 천재식, 주수경,
소속 상세정보
오충훈 ( Oh Chung-Hun ) - 단국대학교 치과대학
이정구 ( Rhee Chung-Ku ) - 단국대학교 의과대학
천재식 ( Chun Gae-Sig ) - 단국대학교 치과대학 구강생리학교실
주수경 ( ) - 단국대학교
KMID : 0829220050290020138
Abstract
We conducted a series of in vitro experiments to evaluate the efficiency of photodynamic therapy on head and neck cancer cell using hydroxybacteriochlorine from photosynthetic bacteria. We tested the cytotoxicity of the hydroxybacteriochlorine by MTT assay and observed the cell death pattern(apoptosis or necrosis) after PDT by hoechst 33342 and propidium iodide staining methods. IC50 value of the hydroxybacteriochlorine was 0.22㎍/㎖. At higher doses of hydroxybacteriochlorine (> 0.6㎍/㎖), cancer cells died exclusively by necrosis after PDT. By contrast, at IC50 value, hydroxybacteriochlorine induced cancer cell to undergo apoptotic cell death. The induction begins approximately 6 hours after PDT. We investigates intracellular localization of hydroxybacteriochlorine by head & neck cancer cell via confocal laser scanning microscopy. Head & neck cancer cells dual-stained with hydroxybacteriochlorine and a panel of organelle-specific fluorescence probes (Mitotracker, Lysotracker, ER-Tracker) revealed an intracellular fluorescence distribution restricted to cytoplasmic compartments with no detectable fluorescence in the nucleus. Confocal images of cells containing hydroxybacteriochlorine were never overlap in subcellular organelle fluorescence when digitally overlayed with the organelle-specific fluorescence probe images of the same cells. These results demonstrated that the hydroxybacteriochlorine may have a function as a photosensitizer.
키워드
Photodynamic therapy;Photosynthetic bacteria;Hydroxybacteriochlorine;Mitotracker;Lysotracker;ER- Tracker
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